BS ISO 3064:2015 pdf download – Essential oil of petitgrain, Paraguayan type (Citrus aurantium L. var. Paraguay (syn. Citrus aurantium var. bigaradia Hook f.))
This International Standard specifies certain characteristics of the essential oil of petitgrain, Paraguayan type (Citrus aurantium L. var. Paraguay (syn. Citrus aurantium var. bigaradia Hook f.)), intended to facilitate the assessment of its quality.
2 Normative references
The following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.
ISO/TS 210, Essential oils — General rules for packaging, conditioning and storage
ISO/TS 211, Essential oils — General rules for labelling and marking of containers
ISO 212, Essential oils — Sampling
ISO 279, Essential oils — Determination of relative density at 20 °C — Reference method
ISO 280, Essential oils — Determination of refractive index
ISO 592, Essential oils — Determination of optical rotation
ISO 875, Essential oils — Evaluation of miscibility in ethanol
ISO 1242, Essential oils — Determination of acid value
ISO 11024 (all parts), Essential oils — General guidance on chromatographic profiles
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
essential oil of petitgrain
essential oil obtained by steam distillation of only leaves and twigs of Citrus aurantium L. var. Paraguay (syn. Citrus aurantium var. bigaradia Hook f.), mainly cultivated in Paraguay, of the Rutaceae family
Note 1 to entry: For information on the CAS number, see ISO/TR 21092. 
Colourless to pale yellow.
Fresh, green, and flowery.
4.4 Relative density, d 20
4.5 Refractive index at 20 °C
4.6 Optical rotation at 20 °C
Between –5° and –1°.
4.7 Miscibility in ethanol 70 % (volume fraction) at 20 °C
It shall not be necessary to use more than 3,5 volumes of ethanol 70 % (volume fraction) to obtain a clear solution with one volume of essential oil.
4.8 Acid value
4.9? Chromatographic? profile
Carry out the analysis of the essential oil by gas chromatography. Identify in the chromatogram obtained,the representative and characteristic components shown in Table 1. The proportions of these components,indicated by the integrator, shall be as shown in Table 1. This constitutes the chromatographic profile of the essential oil.
Information concerning the flashpoint is given in Annex B.
Sampling shall be performed in accordance with ISO 212.
Minimum volume of the test sample: 25 ml.
NOTE This volume makes it possible for each of the tests specified in this International Standard to be carried out at least once.
6 Test methods
6.1 Relative density, d 20
Determine the relative density in accordance with ISO 279.
6.2 Refractive index at 20 °C
Determine the refractive index in accordance with ISO 280.
6.3 Optical rotation at 20 °C
Determine the optical rotation in accordance with ISO 592.
6.4 Miscibility in ethanol 70 % (volume fraction) at 20 °C
Determine the miscibility in accordance with ISO 875.
6.5 Acid value
Determine the acid value in accordance with ISO 1242.
6.6? Chromatographic profile
Determine the chromatographic profile in accordance with ISO 11024.
7 Packaging, labelling, marking, and storage
These items shall be in accordance with ISO/TS 210 and ISO/TS 211.BS ISO 3064 pdf download.