BS ISO 22195-5:2021 pdf download – Textiles — Determination of index ingredient from coloured textile Part 5: Lac This document specifies a test method which identifies the index ingredient chemical included in coloured fabric with lac. Lac can be applied to both natural fibre and man-made fibre. 2 Normative references The following documents are referred to in the text in such a way that some or all of their content constitutes requirements of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO 3696, Water for analytical laboratory use — Specification and test methods 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. ISO and IEC maintain terminology databases for use in standardization at the following addresses: — ISO Online browsing platform: available at https://www.iso.org/obp — IEC Electropedia: available at https://www.electropedia.org/ 3.1 lac scarlet resinous secretion of a number of species of lac insects, of which the most commonly cultivated is Kerria lacca Note 1 to entry: Lac is the common name of insect Kerria lacca. Thousands of lac insects colonize the branches of the host trees and secrete the resinous pigment. The coated branches of the host trees are cut and harvested as stick lac. This stick contains shellac. The spend of shellac extract is used for extraction of lac colorant. 3.2 coloured expressing of colours to textiles by dyeing, printing or coating 3.3 natural colourant materials obtained from plants, wood, rocks, soil, insects or any other thing existing on earth without any chemical reaction adopted before colouring of textiles 4 Principle The identification of natural colorant is very important in the scientific examination of the colouring sources of textiles, coloured print paintings, illuminated manuscripts and other works where natural colorants are used. Natural colourants are usually composed of several phyto chemicals. Each colourant Filter about 1 ml of the extracted solution into a HPLC vial using disposable syringe (6.6) equipped with a membrane filter (6.4). 7.3 Analysis The detection and qualification of laccaic acid C is conducted using HPLC with PDA detector (6.7). The recommendable chromatographic conditions are given in Annex A. Confirm that the retention time and the maximum absorption wavelength of the catechin standard match, or the spectrum matches. 7.4 Qualification of laccaic acid C Comparison between analyses of standard and sample through 7.3...
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