BS ISO 21160:2018 pdf download – Cigarettes — Determination of selected carbonyls in the mainstream smoke of cigarettes — Method using high performance liquid chromatography

BS ISO 21160:2018 pdf download – Cigarettes — Determination of selected carbonyls in the mainstream smoke of cigarettes — Method using high performance liquid chromatography 5 Apparatus The usual laboratory apparatus for use in preparation of samples, solutions and standards and, in particular, the following. 5.1 Equipment for conditioning of tobacco products. 5.2 Equipment for butt length marking. 5.3 Equipment for smoking of tobacco products, complying with ISO 3308. 5.4 Impingers for trapping mainstream smoke. 5.5 Erlenmeyer flasks, of capacities 150 ml, with ground glass stoppers, (or equivalent for combining impinger solutions). 5.6 Polyvinylchloride (PVC) tubing, suitable for connection of the trapping system. 5.7 Analytical balance, capable of measuring to four decimal places. 5.8 Amber glass volumetric flasks, of capacities 10 ml, 25 ml, 200 ml, 1 l and 2 l. 5.9 Glass micropipettes, of capacities 50 µl, 100 µl, 150 µl, 300 µl, 400 µl, 500 µl, 800 µl, 1 000 µl and 2 000 µl. 5.10 Volumetric pipettes, of capacities 1 ml, 2 ml, 5 ml, 6 ml, 7 ml, 8 ml and 20 ml. 5.11 Glass graduated measuring cylinders, of capacities 25 ml, 50 ml and 100 ml. 5.12 Dispenser, capable of delivering 35 ml. 5.13 Hot plate/stirrer. 5.14 Syringe filter, 0,45 µm PVDF or equivalent. 5.15 Disposable syringes, 5 ml. 5.16 Disposable glass Pasteur pipettes. 5.17 Rubber bulbs. 5.18 Autosampler vials, caps and PTFE faced septa. 5.19 HPLC system, consisting of: — tertiary gradient pump; — auto-sampler with appropriate sampling loop; — UV and/or DAD detector; — data collection system; — LC column: 250 mm × 4 mm, 100 Å, Reversed Phase (RP) 18e (5 µm), or equivalent; — disposable guard column: 4 mm × 4 mm RP 18e (5 µm), or equivalent; — vacuum filter; — amber glass bottles 1 l and 4 l; — desiccator; — degasser (optional). 6 Reagents 6.1 Acetonitrile, MeCN, HPLC grade. 6.2 Isopropanol, IPA, HPLC grade. 6.3 Ethyl acetate, HPLC grade. 6.4 Tetrahydrofuran, THF, HPLC grade. 6.5 Ethanol, HPLC grade. 6.6 Phosphoric acid, 85 %. 6.7 Deionized water, resistivity > 18,0 MΩ.cm at 25 °C. 6.8 Formaldehyde-DNPH, min. 99 %. 6.9 Acetaldehyde-DNPH, min. 99 %. 6.10 Acetone-DNPH, min. 99 %. 6.11 Acrolein-DNPH, min. 99 %. 6.12 Propionaldehyde-DNPH, min. 98 %. 6.13 Crotonaldehyde-DNPH, min. 99 %. 6.14 2-Butanone-DNPH, min. 98 %; methyl ethyl ketone-DNPH derivative. 6.15 n-Butyraldehyde-DNPH, min. 99 %. 6.16 Tris-(hydroxymethyl)-aminomethane, ACS reagent grade 1) . 6.17 2,4-Dinitrophenylhydrazine (DNPH). 6.18...