BS ISO 5058-1:2021 pdf download – Biotechnology — Genome editing Part 1: Vocabulary This document defines terms related to genome editing technology. This document is applicable to general use of genome editing across species. 2 Normative references There are no normative references in this document. 3? Terms? and? definitions ISO and IEC maintain terminological databases for use in standardization at the following addresses: — ISO Online browsing platform: available at https:// www .iso .org/ obp — IEC Electropedia: available at http:// www .electropedia .org/ 3.1 Genome editing concepts 3.1.1 gene editing techniques for genome engineering (3.1.3) that involve nucleic acid damage, repair mechanisms, eplication and/or recombination for incorporating site-specific modification(s) into a gene or genes Note 1 to entry: Gene editing is a subclass of genome editing (3.1.2). Note 2 to entry: There are various genome editing tools (see 3.2 and Figure 1). 3.1.2 genome editing techniques for genome engineering (3.1.3) that involve nucleic acid damage, repair mechanisms,replication and/or recombination for incorporating site-specific modification(s) into a genomic DNA Note 1 to entry: Gene editing (3.1.1) is a subclass of genome editing. Note 2 to entry: There are various genome editing tools (see 3.2 and Figure 1). 3.1.3 genome engineering process of introducing intentional changes to genomic nucleic acid Note 1 to entry: Gene editing (3.1.1) and genome editing (3.1.2) are techniques used in genome engineering. 3.1.4 off-target genome editing off-target genomic position and/or nucleic acid sequence distinct from the target (3.1.6) EXAMPLE Off-target binding, off-target cleavage, off-target edit, off-target sequence change. Note 1 to entry: An off-target edit is an example of an unintended edit (3.3.7). 3.1.5 specificity genome editing target specificity extent to which an editing agent or procedure acts only on its intended target (3.1.6) Note 1 to entry: When using this term, the procedure is defined, the intended target is defined, the action or outcome is measured and reported, and limits of detection are reported. 3.1.6 target genome editing target nucleic acid sequence subject to intentional binding, modification and/or cleavage during a genome editing (3.1.2) process Note 1 to entry: See also off-target (3.1.4), Cas nuclease target site (3.2.2.2), meganuclease target site (3.2.3.4), megaTAL target site (3.2.4.3), TALEN target site (3.2.5.4) and ZFN target site (3.2.6.5). 3.2 Genome editing tools 3.2.1 General 3.2.1.1 repair template nucleic acid sequence used to direct cellular DNA repair pathways to incorporate specific DNA sequence changes at or near a target (3.1.6)...
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